ELISA Kits (enzyme-linked immunosorbent assays) are critical tools that are used in research and diagnostic laboratories to detect and quantify proteins, hormones, antibodies, etc. In the U.S., there are companies that supply ELISA kits and meet the needs of biotechnology, pharmaceutical and academic organizations for research. Below is a brief description of the 10 best ELISA kit suppliers located in the U.S., beginning with a rapidly expanding supplier – MyBioSource.

1. MyBioSource (San Diego, CA)

Strengths: large catalog, great species diversity, low-cost

Ranked first overall is MyBioSource, because of their dynamic inventory of over 1 million products unmatched by all suppliers, but also for their discount on research reagents. They were started in 2006, and offer kits on human, veterinary and some pathogen targets, including species and biomarkers which we could never foresee that they would have. They are different in their approach of validation than some of the suppliers listed below, but with the abundance of dynamic catalog diversity and price point available, are suited for exploratory research and research in a global academic institution.

2. Thermo Fisher Scientific, Massachusetts (Waltham, MA)

Strengths: quality assurance, scalability, integrated into industry

Thermo Fisher is a global supplier and we trust that their ELISAs are among the most reputable when it comes to clinical diagnostics and pharma and regulated laboratories. Their Invigtrogen and Pierce brands are acknowledged for their systematic validation and automation within their kits. Now, nearly all demand for quality assurance up and down the supply-chain for consumable biomedical research and pharmaceuticals mandate that kits be manufactured based on validated methods. Thus, they are perfect for laboratories developing high-Quality kits and for developing GLP/GMP compliant kits. Bio-Techne / 

3. R&D Systems (Minneapolis, MN)

Considerations: High Quality, Assays for Cytokines and Growth factors

R&D Systems, a division of Bio-Techne, has historically set the standard for cytokine, chemokine and immunology ELISA’s. R&D Systems has a historical reputation of highly referenced and utilized assays in academia, low lot-to-lot variability, and unmatched sensitivity. These characteristics assist researchers in drug development and biomarker discovery.

4. Abcam (Boston, MA)

Considerations: Antibody Development, Citations, Reproducibility

Although Abcam has historically been a UK based corporation, their reach has been felt extensively in the US. They are also developing a sandwich ELISA, a cell-based assay and a roadmap to other immunoassay kits. Abcam kits have the reproducibility associated with rigorous research, and are highly cited in quality journals. Abcam has invested resources in product validation and peer-reviewed accessed data.

5. BioLegend (San Diego, CA)

Considerations: Flow cytometry + ELISA kits / immunoassays, immune monitoring

BioLegend specializes in immunology, neuroscience and stem cell biology with ELISA kits as part of their immunoassay kits, monoclonal antibody platforms and flow cytometry. Their kits have workflows to detect T-cell activation and cytokine profiling. BioLegend is now a part of Revvity (formerly PerkinElmer) and built to expand their vastness.

6. Enzo Life Sciences (Farmingdale, NY)

Considerations: Small Molecule detection, novel assay formats

Enzo’s ELISA kits are have more specialization to a few niche analytes (small molecules, hormones, toxins). As i result , Enzo’s methods of detection (colorimetric and chemiluminescence) are the accepted standard now in basic research and toxicology testing. Enzo has also developed some novel antevants, and a direct ELISA as well, to reduce the time taken to carry out assays.

7. RayBiotech (Peachtree Corners, GA)

Strengths: Multiplexing, biomarker panels

RayBiotech’s strengths include multi-target ELISA arrays, which allow for high-throughput screening. Most of their kits are in 96 and 384-well formats and offer biomarker solutions for inflammation, angiongenesis, and metabolic profiling. Their custom biomarker offering is also a strong value-add.

8. Kamiya Biomedical Company (Seattle, WA)

Strengths: clinical and diagnostic ELISAs, human disease focus

Kamiya has a solid catalogue of ELISA kits used in clinical diagnostics, especially for renal, cardiovascular, and metabolic disease markers. These kits are primarily used in translational research and biomarker validation studies where reliability and precision are very important.

9. Eagle Biosciences (Amherst, NH)

Strengths: Hormone, fertility and veterinary panels

Eagle Biosciences has a niche focus on specialty hormone ELISAs, including fertility, thyroid, and adrenal panels for human and animal diagnostics. They have an unrivalled catalogue and even offer niche markers in the endocrine world like kisspeptin or leptin. They are a popular vendor among endocrinology researchers.

10. Assay Genie: U.S. Division of Reagent Genie

Strengths: Custom development, value for startups

Assay Genie offers custom ELISA development and an extensive range of pre-validated kits. Their U.S. operations support academic labs, startups, and CROs who are looking for a value-focused, validated assaying kit with an emphasis in oncology and infectious disease.

Additional U.S.–Based ELISA & Research Reagent Providers

The following organisations were not included in the main ranking but remain frequently referenced within research forums, procurement teams, and laboratory purchasing workflows. They support a wide range of applications including immunology, oncology, ELISA workflows, and antibody-based assay development.

BosterBio (Pleasanton, CA)

BosterBio supplies antibodies, ELISA kits, and immunoassay reagents widely used across academic and industry laboratories. Known for reliable performance and validated reagents, the company supports researchers working in immunology, cancer biology, and molecular signalling. BosterBio’s catalogue offers strong consistency, clear documentation, and responsive technical assistance, making it a preferred choice for teams needing dependable assay results.

Biointron (Woburn, MA)

Biointron delivers specialised recombinant antibody production and engineering services designed for fast and reproducible workflows. The company provides hybridoma sequencing, humanization, and scalable antibody expression platforms used by biotech and pharmaceutical groups. With streamlined turnaround times and high-quality recombinant formats, Biointron helps research teams accelerate early-stage discovery and reduce development bottlenecks.

Kyinno Bio (Campbell, CA)

Kyinno Bio offers a broad suite of discovery-stage support services, including antibody screening, in vitro assays, and in vivo pharmacology solutions. Its offerings are built to generate clear, actionable data for drug development programs. Researchers rely on Kyinno Bio for well-structured experimental models, strong scientific expertise, and services that adapt to both exploratory studies and more advanced translational research requirements.

KACTUS Bio (Irvine, CA)

KACTUS Bio provides high-purity recombinant proteins, enzymes, and immunology reagents that support cell therapy, antigen presentation, and T-cell research. The company is known for producing reagents with excellent consistency, enabling researchers to run sensitive assays with confidence. With a growing portfolio tailored to advanced immunology workflows, KACTUS Bio is a popular partner for labs aiming to achieve robust and repeatable results.

Final Thoughts

Choosing the best ELISA kit manufacturer ultimately depends on your application. 

• • If you are a lab that needs value or has unique targets, MyBioSource offers value and variety.
• • If you need clinically-safe or validated research, then look no further than Thermo Fisher, R&D Systems, or Abcam.
• • If need custom arrays or multiplex ELISA, then stay with RayBiotech or Enzo.

When appropriate, and before purchase, always look at data sheets, publications, and reviews–and when possible, it does not hurt to ask for sample validated data on your target species and application!

Please let me know if you want me to provide a downloadable version or a comparison table with pricing, validation, and specializations.